Underwater transect surveys have conventionally been conducted by dive teams, a method that, while effective, often comes with logistical challenges and higher costs. However, with the advent of advanced Underwater Remote Operated Vehicle (ROV) technology, these surveys can now be carried out more efficiently and economically. ROVs present a practical alternative to traditional diving methods, offering several advantages in conducting underwater transect surveys. This guide will dive into the use of ROVs for conducting these surveys, outlining the steps and considerations involved in leveraging this innovative technology for underwater ecological studies.